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. 2020 Feb 25;8(3):42. doi: 10.3390/biomedicines8030042

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of anticoagulants on intra-platelet free Ca²⁺ concentration. Pure-PRP samples were prepared from whole-blood samples stored for 24 h. (a) Representative histograms of Fluo4⁺ platelets. (b) Median fluorescence intensities of platelets. (c) Percentages of Fluo4⁺ cells. n = 6.