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Multiplex PCR of amplified phylogenetic groups. Lane (1): DNA marker 100 bp. Lane (2, 3, 6, 7, and 9): chuA gene (279 bp) and yjaA gene (211 bp) and TspE4.C2 (152 bp). Lane (4): chuA gene (279 bp) and TspE4.C2 (152 bp). Lane (5): yjaA gene (211 bp. Lane (8): yjaA gene (211 bp) and TspE4.C2 (152 bp). Lane (10): Positive control (Escherichia coli ATCC25922). Lane (11): Negative control.
