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Knockdown of ROCK2 decreases MMP24 expression. Cells were infected with ROCK2 shRNA-expressing retrovirus. Confocal images of cells stained for YAP (green) and DAPI (blue) are shown (A). The expression of ROCK2 (B), MMP24 (C), CTGF (D), and MMP7 (E) were evaluated by quantitative real-time PCR. Each bar represents the mean ± S.D.; n = 3. Asterisks represent p < 0.005. Double asterisks represent p < 0.01.
