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. 2020 Mar 16;117(14):7941–7949. doi: 10.1073/pnas.1912022117

Fig. 4.

Fig. 4.

Asynchronous cross-fostering results in translocation of gut-residing E. coli. (A) Schematic of asynchronous cross-fostering (ACF) protocol. (B) Concentration of EGF in stool of conventionally reared (Con, black triangle), SCF (green circles), or ACF (blue squares) pups. (C) CFUs in stool 3 d following gavage of 2 × 105 CFUs of E. coli C1nalR (blue) or BSI-AnalR (red) in SCF and ACF mice. (D) CFUs in mesenteric lymph node (MLN), spleen, and liver 3 d following gavage of 2 × 105 CFUs of E. coli C1nalR (blue) or BSI-AnalR (red) in SCF and ACF mice. (E) Survival of SCF and ACF mice following gavage of 2 × 105 CFUs of E. coli C1nalR or BSI-AnalR in SCF and ACF mice. n = 5 in B–D. n = 15 mice from three independent litters per group. Individual data points plotted in B–D with mean and SD plotted per group. Statistics used: two-way ANOVA (B), Mann–Whitney (D), Kaplan–Meier (E), *denotes statistical significance, P < 0.05 or less.