Fig. 4.

Pum1; Pum2 DKO ESCs have abnormal expression of key germ-layer differentiation markers in EBs and teratomas. (A) Morphological comparison of wild-type and mutant EBs on day 2, day 6, day 10, and day 12. (Scale bars, 250 μm.) (B–J) Relative expression of markers for pluripotency and three germ layers as quantified by qRT-PCR and normalized over GAPDH mRNA. Results are presented as mean ± SD. t-test: *P < 0.05, **P < 0.01, ***P < 0.001. (K) H&E staining of teratoma histological sections: (a) ectoderm-like pattern; (b) mesoderm-like pattern; (c) endoderm-like pattern.