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. 2020 Mar 20;117(14):7851–7862. doi: 10.1073/pnas.1916471117

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Fig. 6. — Pum1 and Pum2 regulate the translation of many mRNAs in ESCs. (A) The metaplot of ribosomal P-site mapping around the start and stop codons of annotated protein-coding regions in wild-type ESCs. The trinucleotide periodicity feature of ribosomal protection assay is clearly evident. +20-bp, −40-bp region relative to start codon is displayed for translation starting region; +40-bp, −20-bp region relative to end codon is displayed for translation stopping region. (B) The metaplot of P-site mapping around the start and the stop codon of annotated CDs in wild-type (black and red) and Pum double-mutant (blue, green, and orange) ESCs. Reads were normalized to sequencing depth. (C) Venn diagrams of translationally increased and decreased genes in Pum1^−/−, Pum2^−/−, and Pum double mutants compared to wild type. (D–E) The proportion of direct target and PRE-containing mRNA isoforms that are in translationally increased (D) or decreased (E) in Pum1^−/−, Pum2^−/−, and Pum double mutants compared to wild type.