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. 2020 Mar 20;117(14):7851–7862. doi: 10.1073/pnas.1916471117

Fig. 7.

Fig. 7.

A model of Pumilio function in ESCs and embryogenesis. During early embryogenesis, pluripotent stem cells (blue) differentiate into daughter cells (purple). Pum2 promotes ESC self-renewal while Pum1 promotes ESC differentiation; both negatively regulate each other’s expression. In the Pum1 knockout, Pum2 is overexpressed, and pluripotency markers Nanog, Oct4, and Sox2 are elevated. In the Pum2 knockout, Pum1 is overexpressed; Nanog levels are decreased while there is early expression of the differentiation markers Gata6, FoxA2, Brachyury, GSC, and FGF5. In the Pum1/2 double knockout there are decreased levels of both pluripotency and differentiation markers, delayed development by e3.5, and defective germ-layer development that leads to embryonic lethality by e8.5. Overall, this model highlights the role of Pum1 in differentiation, Pum2 in self-renewal, and both collectively for normal embryogenesis.