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Cofiltration of Gyp1-46 protein and fusion inhibition activity. Affinity-purified recombinant Gyp1-46 (1 mL, 138 μM; ref. 35) was applied to an 0.7 × 45 cm column of Superdex 200 (prep grade) in RB150 at 4 °C, eluted by gravity, and 70 fractions of 0.5 mL were collected. Each was assayed for protein by Bradford and, after fivefold dilution in RB150, for inhibition of fusion of proteoliposomes bearing Ypt7-tm charged with GTP.
