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. 2020 Mar 25;117(14):7739–7744. doi: 10.1073/pnas.2000923117

Fig. 5.

Fig. 5.

Ypt7-pr on R-proteoliposomes must bear GTP for optimal fusion with HOPS. (A–F) Fusion reactions contained proteoliposomes bearing R- or QaQb-SNAREs at 1:16,000 SNARE to lipid molar ratio with 1:8,000 protein to lipid molar ratio of either Ypt7-pr (A–C) or Ypt7-tm (D–F). R-proteoliposomes were charged with either no GXP (A and D), GDP (B and E) or GTP (C and F) and QaQb-proteoliposomes were charged with No GXP (black lines), GDP (red lines), or GTP (green lines). Content mixing was assayed in the presence of 50 nM HOPS and 100 nM Qc by measuring FRET between luminal Cy5 and phycoerythrin for 30 min at 27 °C. Kinetic curves of content mixing assays in this figure are representative of n ≥ 3 experiments; see SI Appendix, Fig. S5.