Fig. 4.

Biosynthetic precursors are synthesized on demand during cell division. Frequency of metabolites across five main superpathways: (A) amino acid metabolism, (B) nucleotide metabolism, (C) carbohydrate and energy metabolism, (D) cofactor and vitamin metabolism, and (E) lipid metabolism. Metabolites were binned according to which cell cycle phase they have peak abundance in, and which submetabolic pathway they belong to. Abundance values were calculated by averaging replicate abundance in each cell cycle phase (G1, n = 12; S phase, n = 9; G2/M, n = 9), with the cell cycle phase that contained the maximum abundance value for a given metabolite increasing in metabolite frequency in each bar plot. Annotation by Metabolon, Inc. was used as a reference for superpathway and subpathway classification.