Table 4.
Activity of topical application of the solvent fraction extract ointment of the flower of Hagenia abyssinica on wound contraction of excision wound model in mice.
| Wound area (mm2) (mean ± SEM) (% contraction) | ||||||||
|---|---|---|---|---|---|---|---|---|
| Day | SO | NF | 5% AQFO | 10% AQFO | 5% CHFO | 10% CHFO | 5% EAFO | 10% EAFO |
| 0 | 312.67 ± 1.09 | 311.33 ± 0.71 | 312.25 ± 0.95 | 312.67 ± 0.76 | 312.67 ± 0.56 | 311.83 ± 0.87 | 312.83 ± 0.79 | 312.00 ± 0.77 |
| 2 | 304.17 ± 3.49 (2.72%) | 288.54 ± 2.96 (7.29%)a1 | 296.88 ± 2.68 (4.92%) | 288.54 ± 2.98 (8.72%)a1 | 300 ± 2.28 (4.05%) | 296.88 ± 3.52 (4.79) | 293.75 ± 3.23 (6.10%) | 286.46 ± 2.98 (8.19%)a2 |
| 4 | 286.46 ± 3.49 (8.38%) | 251.04 ± 2.98 (19.37%)a2 | 271.86 ± 2.77 (12.94%)a1d3 | 259.36 ± 3.52 (17.05%)a2 | 276.04 ± 2.51 (11.72%)b3c2d3e1 | 270.83 ± 3.09 (13.15)a1b2d3 | 261.46 ± 2.98 (16.42%)a2d1 | 247.92 ± 3.09 (20.54%)a3 |
| 6 | 201.04 ± 2.98 (35.70%) | 173.96 ± 3.78 (44.12%)a3 | 184.38 ± 3.52 (40.95%)a1d3 | 170.83 ± 2.08 (45.36%)a3 | 189.58 ± 4.17 (39.37%)b1c2d3 | 185.42 ± 2.64 (40.54)a1c1d3 | 186.46 ± 2.98 (40.40%)a1c1d3 | 162.50 ± 2.28 (47.92%)a3 |
| 8 | 98.96 ± 1.92 (68.35%) | 75.00 ± 3.76 (75.91%)a3 | 81.25 ± 2.28 (73.98%)a2c1d3 | 67.71 ± 2.98 (78.34%)a3 | 81.25 ± 2.28 (74.01%)a1c1d3 | 80.21 ± 2.98 (74.28)a1d3 | 78.13 ± 2.68 (75.02%)a2d3 | 55.21 ± 2.98 (82.30%)a3b3 |
| 10 | 71.88 ± 2.68 (77.01%) | 35.42 ± 3.09 (88.62%)a3 | 51.04 ± 2.28 (83.65%)a2b1c2d3 | 31.25 ± 2.28 (90.01%)a3 | 55.21 ± 1.92 (82.34%)a1b2c3d3e1 | 39.58 ± 2.08 (87.31)a3d2 | 41.69 ± 4.47 (86.67%)a3d3 | 19.79 ± 3.76 (93.66%)a3b1 |
| 12 | 43.75 ± 2.68 (86.01%) | 10.42 ± 3.49 (96.65%)a3 | 26.04 ± 2.98 (91.66%)a2b2d2 | 16.67 ± 2.64 (94.67%)a3 | 30.21 ± 3.76 (90.34%)a1b3c1d3 | 22.92 ± 2.08 (92.65)a3d2 | 20.33 ± 1.94 (93.50%)a3d1 | 9.37 ± 2.68 (97.66%)a3 |
| 14 | 25.00 ± 2.28 (92.00%) | 3.13 ± 2.13 (100%) | 10.38 ± 3.48 (96.68%)a2 | 2.08 ± 1.32 (99.33%)a3 | 13.54 ± 2.98 (95.67%)a1b2c1d2 | 9.38 ± 3.13 (97.00)a2 | 7.33 ± 1.92 (97.66%)a3 | 1.04 ± 1.04 (99.67%)a3 |
| 16 | 13.54 ± 2.98 (95.67%) | 0 (100%) | 3.13 ± 2.13 (99.00%)a2 | 0 (100%)a3 | 3.13 ± 2.13 (99.00%)a2 | 1.08 ± 1.04 (99.65)a3 | 0 (100%)a3 | 0 (100%)a3 |
Note. Values are expressed as mean ± SEM (n = 6 mice in each group) and analyzed by one-way ANOVA followed by the post hoc Tukey test; acompared to the negative control; bcompared to positive control; ccompared to 10% AQFO; dcompared to 10% EAFO; ecompared to 5% EAFO; 1P < 0.05; 2P < 0.01; 3P < 0.001; AQFO = aqueous fraction ointment; CHFO = chloroform fraction ointment; EAFO = ethylacetate fraction ointment; NF = nitrofurazone 0.2% ointment; SO = simple ointment.