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. 2020 Mar 27;2020:9847579. doi: 10.1155/2020/9847579

Table 2.

Protein concentration analyses of cultured airway epithelial (AE) cells. The concentrations of coagulation factor 3 and angiopoietin 2 in cell culture media were measured using ELISAs. AE cells were cultured for three days either alone or in coculture with mesenchymal stem cells (MSCs) in inserts. Mesenchymal stem cells were obtained from the bone marrow (BM) or adipose tissue (AD). Concentrations were measured in culture medium that had no addition of any cells and were used subsequently as blank control and subtracted from all other measurements, which are given as blanked protein concentrations in pg/mL. Data are given as means ± standard deviation. ∗ indicates statistically significant difference between AE culture alone and coculture.

Coagulation factor 3 Angiopoietin 2
Medium blank 2.2 ± 0.4 51.9 ± 0.8
BM MSCs 0.0 ± 0.5 −4.6 ± 2.7
AD MSCs 3.2 ± 4.7 2.4 ± 7.8
AE 600.5 ± 182.6 −15.8 ± 1.6
Total AE in BM MSC coculture 612.6 ± 154.7 −17.1 ± 4.6
Net AE in BM MSC coculture 612.6 ± 154.8 −12.5 ± 3.5
Total AE in AD MSC coculture 553.3 ± 120.8 1.5 ± 7.5
Net AE in AD MSC coculture 550.1 ± 120.9 −0.9 ± 4.4