Fig. 3.

Chromosome rearrangements are mediated via the gRNA cleavage site. (A and B) Simplified outline maps depicting the chromosomes that underwent translocation in VYD135 (A) and VYD136 (B). Black semicircles, telomeres; red semicircles, de novo telomeres; narrow bands, centromeres; shaded box on chromosome 5, MAT locus. (C) Nanopore reads mapping to the wild-type H99 genome revealed the sites of DSB formation or repair junctions at centromeres. The reads either converge on a single site (CEN5, CEN8) or exhibit sequence gaps between sites (CEN10) marking the location of junctions. Red bars indicate the centromeres, whereas the black vertical lines mark the site of gRNA cleavage. Cov, coverage of nanopore reads; Map, mapping of nanopore reads.