Chromosome-shuffled isolates exhibit defects in sexual reproduction. (A) Light microscopy images showing hyphae, basidia, and spore chains in crosses between MATa wild-type strain KN99a and MATα wild-type (H99) and rearranged strains (VYD135 and VYD136). (Scale bar, 100 µm.) (B) Scanning electron microscope (SEM) images depicting a complete or partial sporulation defect when wild-type KN99a was crossed with VYD135 and VYD136, respectively. (Scale bar, 10 µm.)