Figure 9.3.

Cell maintenance.

Adherent cell lines are maintained in flasks or plates and regular medium changes ensure healthy cell propagation. Once ~80% confluency has been reached, cells are enzymatically or mechanically dissociated from their plating substrates. The detached cells can be collected in a Falcon tube and pelleted. With a subset of these cells new cell culture vessels can be seeded, while any remaining cells can be frozen or utilized for downstream experiments.