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. 2017 Apr 7:151–172. doi: 10.1016/B978-0-12-803077-6.00009-6

Table 9.2.

Recommended Equipment for the Cell Culture Laboratory

Equipment Purpose
Biosafety cabinet
  • –

    To create sterile work surface; class II and III recommended
Humid CO2 incubator
  • –

    To provide a physiological environment for cellular growth
Inverted light microscope
  • –

    To assess cell morphology and count cells
Fridge, freezers (−20°C, −80°C), liquid nitrogen storage
  • –

    To store cells, cell material, and culture components
Centrifuge
  • –

    To condense cells
pH meter
  • –

    To determine the correct pH of media components
Pipettes and pipettors
  • –

    To aliquot different volumes
Cell media and supplementary components
  • –

    To culture cells in desirable components
Hemacytometer
  • –

    To count cells, determine growth kinetics and prepare suitable plating densities
Autoclave
  • –

    To sterilize pipettes and other equipment in contact with cells
Vacuum pump
  • –

    To aspirate cell culture medium
Water bath (with adjustable temperature)
  • –

    To warm up cell culture media
Cell culture dishes
  • –

    To culture cells in different formats (e.g., flasks, Petri dishes, 96-well plates)
Containers for waste (biohazardous)
  • –

    To correctly dispose of waste