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. 2020 Mar 25;117(14):7831–7836. doi: 10.1073/pnas.1916452117

Fig. 3.

Fig. 3.

Fibril elongation observed at high spatial and temporal resolution. (A) Successive HS-AFM images of a growing Sup35NM fibril captured at a pixel size of 0.5 nm. (Imaging rate, 0.5 fps; bar, 10 nm.) (B) Time courses of length change of growing Sup35NM fibrils. The different lines indicate different fibrils. Red and blue lines show the time courses of fibril elongation when a Sup35NM sample (0.5 µM) was preincubated for 30 min; green and purple lines show cases when the same sample was preincubated for 90 min. (Inset) Magnified time course taken from the blue line in the period from 270 to 310 s. (Grid interval of y-axis, 1 nm.) (C) Successive HS-AFM images of a growing Sup35NM fibril captured at high temporal resolution. (Imaging rate, 10 fps; bar, 20 nm; pixel size, 1 nm.) (D) Time course of length change of growing Sup35NM fibrils. The different lines correspond to different fibrils. (Inset) Magnified time course taken from the red line in the period from 11 to 14 s. Sup35NM samples (0.5 µM) were preincubated for 30 min. (Grid interval of y-axis, 1 nm.)