Fig. 4.

Host membrane trafficking to Ehrlichiae requires bacterial protein synthesis. RF/6A cells were seeded on coverglasses and infected with E. chaffeensis (Ech). Cells were treated with medium control (CTL, A), 5 μg/mL of oxytetracycline (OTC) at 2 dpi for 4 h (B), or at 1 dpi for 1 d (C), then labeled with DiI (5 μM) for 6 h. After fixation and staining with Hoechst 33342, cells were observed under a DeltaVision microscope. Arrows, E. chaffeensis–containing inclusions. (Scale bars, 10 μm.) (D) Percentage of DiI-labeled membranes of Ehrlichia inclusions among total inclusions were quantified by counting at least 10 cells per group, and results are shown as mean ± SD. Asterisk (*), significantly different from the control group and between OTC treatment time courses (P < 0.01, ANOVA).