Figure 4.

Relative mRNA expression of catecholamine biosynthetic enzyme expression (2^-ΔΔCt). qPCR gene expression of catecholamine biosynthetic enzymes including (a, b) tyrosine hydroxylase (TH), (c, d) dopamine beta-hydroxylase (DBH), and (e, f) phenylethanolamine N-methyltransferase (PNMT) for males and females. Two-way ANOVA (Fisher LSD test): statistical significance is represented as ^∗/†P ≤ 0.05, ^{∗∗/††}P ≤ 0.01, ^{∗∗∗/†††}P ≤ 0.001, and ^{∗∗∗∗/††††}P ≤ 0.0001. Saline-Control and Dex-Control are shown here for reference, and results have been described previously. Data are presented as mean ± SEM. ∗ indicates significance relative to Saline-Control, and † indicates significance relative to (c). N = 4‐6 per group.