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. 2016 Sep 16:241–260. doi: 10.1016/B978-0-12-802168-2.00020-8

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Copyright © 2017 Elsevier Inc. All rights reserved.

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Figure 20.11 — Interleukin-2 (IL-2) mRNA.

Effect of in vitro addition of Zn, Mn, Fe, and Cu on IL-2 and IL-2R mRNAs in elderly subjects. Human MNCs were isolated from zinc-deficient and zinc-sufficient elderly subjects. Either 15-μM Zn (as chloride), Mn (as chloride), Fe (as sulfate), or Cu (as sulfate) was added to isolated MNCs for 24 h, followed by PHA stimulation for another 24 h. Total RNA was extracted, and samples were subjected to RT-PCR analysis. The data shown here are representative of three subjects from each group. Lane one represents MNCs from zinc-deficient subjects. Lanes two to five represent MNCs from zinc-deficient subjects with either Zn, Mn, Fe, or Cu added to the media. Lane six is the MNCs from zinc-sufficient subjects with Zn addition in the media. The results indicate that zinc addition, but not Mn, Fe, and Cu, increased the IL-2 (A, B) and IL-2R (C, D) mRNAs from the MNCs of zinc-deficient subjects (p = .05).

Reproduced from Prasad, A.S., Bao, B., Beck, F.W.J., Sarkar, F.H., 2006. Correction of IL-2 gene expression by in vitro zinc addition to MNC from zinc deficient human subjects: a specific test for zinc deficiency in humans. Transl. Res. 148, 325–333.