Figure 3.

Metabolic heterogeneity within and between islets of Langerhans in a pancreatic tissue section of one mouse (A–E). The column on the left shows multiplex immunostainings after MALDI imaging mass spectrometry. Alpha cells (red) and beta cells (green) are stained with glucagon and insulin, respectively. A tissue fold in the fifth islet (E) was excluded from analyses (dashed). The second and third columns show spatial correlation networks for metabolites in alpha and beta cells, respectively. Nodes and edges represent compounds and their spatial correlation. The networks shown here only include direct neighbors of the glucose 6-phosphate node and edges representing a correlation coefficient of at least 0.7. Scale bar, 150 μm. Abbreviations: adenosine monophosphate (AMP), guanosine monophosphate (GMP), phosphatidic acid (PA), phosphatidylethanolamine (PE), lysophosphatidic acid (LPA), lysophospholipid (LPC), lysophosphatidylinositol (LPI), dihydroxyacetone phosphate (DHAP), glycerophosphoinositol (GroPIns), phosphodimethylethanolamine (P-DME).