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Comparison of H&E and expression of representative markers for cell types, diagnosis and proliferation. Original melanoma MSE and the respective 2D and 3D cell cultures were compared. Pan-CK (tumour cells), CD45 (immune cells) and calretinin (mesothelial cells) positive expression can be visualised in brown stain. Double staining of Melan-A (melanoma diagnostic marker) and MIB-1 (proliferation marker) are detected, respectively, in red and brown stains, scale bar = 100 µm
