Fig. 2.

At P14, there was persisted microglial depletion following HI at P10. a Gating strategy for FACS analysis. After dead cell elimination, first gating was defined as CD11b⁺CD45⁺ while resident microglial cells were defined by CD11b⁺CD45⁺EYFP⁺ status. b Quantification of resident microglial cells in males for each group (DTA⁻ HI group, n = 7; DTA⁺ HI group, n = 5; DTA⁻ sham group, n = 3; DTA⁺ sham group, n = 3). c Quantification of resident microglial cells in females in each group. (DTA⁻ HI group, n = 8; DTA⁺ HI group, n = 3; DTA⁻ sham group, n = 4; DTA⁺ sham group, n = 3) **P < 0.01, ***P < 0.001, ****P < 0.0001; one-way ANOVA followed by the Sidak test. Bars reflect mean ± SD