Fig. 4.

TUNEL and NeuN staining at P13 following HI insult at P10. a Representative image of co-immunofluorescent staining at the striatum level. The scale bar indicates 500 μm. b High magnification of the TUNEL⁺ area at the striatum level in each gender and genotype in the square shown in Fig. 1. Scale bar indicates 20 μm. c Quantification of TUNEL⁺ cells/mm² in the caudoputamen and cerebral cortex. d Comparison of sex differences within the same genotype. The number of TUNEL-positive cells in each interest region at P13 following HI insult at P10 (DTA⁻ male, n = 12; DTA⁺ male, n = 10; DTA⁻ female, n = 6; DTA⁺ female, n = 12) **P < 0.01, ***P < 0.001. For the caudoputamen, two-way ANOVA was performed followed by the Bonferroni multiple comparison test. For the cerebral cortex, the Kruskal-Wallis test was performed followed by Dunn’s multiple comparison test. Bars depict mean ± SD