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. 2020 Apr 10;21:17. doi: 10.1186/s12865-020-00348-x

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Interleukin (IL)-35 concentration in the plasma and IL-35 receptor mRNA relative level in CD14⁺ monocytes in patients with Kawasaki disease (n = 33) and controls (n = 17). Plasma IL-35 concentration was measured by enzyme linked immunosorbent assay. mRNA corresponding to IL-35 receptor subunits, including IL-12Rβ2 and gp130, in purified CD14⁺ monocytes were semi-quantified by real-time reverse transcriptional polymerase chain reaction. a IL-35 concentration was elevated in the plasma from patients with Kawasaki disease when compared with controls. There were no significant differences of (b) IL-12Rβ2 or (c) gp130 mRNA relative level between patients with Kawasaki disease and controls. Student’s t test was used for comparison. Individual level of each subject was shown. The horizon line presented mean, and error bar presented standard deviation