Table VI.

Nutrient Requirements of Mice ^a

Nutrient	Concentration in diet (%)
Protein (as crude protein)	20–25
Fat b	5–12
Fiber	2.5
Carbohydrate	45–60

Estimated Dietary Amino Acid Requirement
Amino acid	Natural-ingredient, open-formula diet (%)c	Purified diet (%)d
Arginine	0.3	−
Histidine	0.2	−
Tyrosine	−	0.12
Isoleucine	0.4	0.2
Leucine	0.7	0.25
Lysine	0.4	0.15
Methionine	0.5	0.3
Phenylalanine	0.4	0.25
Threonine	0.2	0.22
Tryptophan	0.1	0.05
Valine	0.5	0.3

Mineral and Vitamin Concentrations of Adequate Mouse Diets
Mineral	Natural-ingredient, open-formula diet e	Purified dietf	Purified dietg	Chemically defined diet h
Calcium (%)	1.23	0.52	0.81	0.57
Chloride (%)	−	0.16	−	1.03
Magnesium (%)	0.18	0.05	0.073	0.142
Phosphorus (%)	0.99	0.4	0.42	0.57
Potassium (%)	0.85	0.36	0.89	0.40
Sodium (%)	0.36	0.1	0.39	0.38
Sulfur (%)	−	−	m	0.0023
Chromium (mg/kg)	−	2.0	1.9	4.0
Cobalt (mg/kg)	0.7	−	−	0.2
Copper (mg/kg)	16.1	6.0	4.5	12.9
Fluoride (mg/kg)	−	−	−	2.3
Iodine (mg/kg)	1.9	0.2	36.0	3.8
Iron (mg/kg)	255.50	35.0	299.0	47.6
Manganese (mg/kg)	104.0	54.0	50.0	95.2
Molybdenum (mg/kg)	−	−	−	1.55
Selenium (mg/kg)	−	0.1	−	0.076
Vanadium (mg/kg)	−	−	−	0.25
Zinc (mg/kg)	50.3	30.0	31.0	38.0