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Scheme of creation of plasmids used for luciferase assay. DBH Ins allele was cloned into pGL3 Basic between Mlu I and Xho I to generate pGL3 DBH promoter WT. The Ins allele was removed by restriction digestion of the region of Ins allele with two flanking restriction sites (Nhe I and Sac II) and an amplified fragment with Del allele was cloned to generate pGL3 DBH promoter 19bp Del.
DBH: dopamine-β-hydroxylase; PCR: polymerase chain reaction
