Table 1.

Recombinant Proteins Expressed Using Ad Vectors and Yields Obtained

Protein expressed	Position	Serotype	Promoter	Cell linea	Yieldb	Reference
Human α1antitrypsinc	El	Ad5	MLP + TPL	HeLa	6 μg/ml/106 cells	Gilardi et al. (1990)
Human α1-antitrypsnvc	El	Ad5	MLP	HUVEC	0.3–0.6 μg/106 cells/day	Lemarchand et al. (1992)
β-Galactosidase	El	Ad5	CMV	MRC5	27 μg/6 cm dishd	Wilkinson and Akrigs (1992)
Luciferase	E3	Ad5e	SV40 early	HeLa	20 μg/106 cells	Mittal et al. (1993)
PTP1C protein tyrosine phosphatase	El	Ad5	MLP + TPL	293 S	90 mg/liter	Garnier et al. (1994)
PTP1C protein tyrosine phosphatase	El	Ad5	MLP + TPL	293	100 μg/150 mm dish	Zhao et al. (1993)
HBsAgc	El	Ad5	E1a	Vero	0.5–1 μg/106 cells	Ballay et al. (1985)
HBsAg	E4/ITR	Ad4e, Ad7e	MLP + TPL	A549	3–4 μg/106 cells	Mason et al. (1990)
HbsAgc and IL-6c	E4/ITR, E3	Ad7e	MLP + TPL, E3	A549	8–10 μg/106 cells, 0.1–0.2 μg/106 cells	Lindley et al. (1994)
Hepatitis B precore antigen,c HbSAgc	E3, E4/ITR	Ad7e	E3, MLP + TPL	A549	1.4 μg, 6 μg /106 cells	Ye et al. (1991)
Measles virus N protein	El	Ad5	CMV	MRC5	20% of soluble cell proteinb	Fooks et al. (1995)
Porcine respiratory coronavirus spike glycoprotein	E3	Ad5	E3	ST	33 μg/106 cells	Callebaut and Pensaert (1995)
HTV-1 env	E3	Ad5e	E3	HeLaS	1 mg/liter of culture	Dewar et al. (1989)
HIV-1 env	E4/ITR	Ad7e	MLP + TPL	A549	4 μg/106 cells	Chanda et al. (1990)
Polyomavirus middle T antigen	El	Ad5	MLP + TPL	293	100 μg/100-mm dish	Schaffhausen et al.
Murine IL-12 (both subunits)c	El, E3	Ad5	CMV, CMV	293	42,000 U/106 cells/day	Bramson et al. (1996)
Soluble ciliary neurotrophic factorc	El	Ad5	RSV LTR	1° rat astrocytes	120 pg/106 cells/hr	Smith et al. (1996)
Neurotrophin-3c	El	Ad5	RSV LTR	1° rat astrocytes	350 pg/106 cells/hr	Smith et al. (1996)
Fibroblast growth factor 4	El	Ad5	Sr α	Dami	2–3 μg/106 cells	Konishi et al. (1996)
Human IL-2c	El	Ad5	CMV	Various human	1–2 μg/106 cells	Addison et al. (1995)
VEGFc	El	Ad5	CMV	HUVEC	2.2 μg/106 cells/day	Mühlhauser et al. (1995)
GM-CSFc	El	Ad5	CMV	293	8 μg/106 cells	Xing et al. (1996)

^aHeLa, human cervical carcinoma cells; A549, human lung carcinoma; HUVEC, primary human vascular endothelial cells; MRC5, human fibroblasts; 293, Ad5-transformed human embryonic kidney cell line; 293S, suspension subline of 293; Vero, monkey kidney cell line; ST, swine testicle cell line; Dami, human megakaryocytic cell line.

^bYields reported by the authors were converted to μg recombinant protein/10⁶ cells.

^cSecreted to medium.

^dForskolin induced.

^eNondefective vector.