Table 2.

Advantages and Disadvantages of Adenoviral Vectors

Advantages	Disadvantages
Versatility; recombinant protein expressed from same vector both in vitro and in vivo	Complexity of recombinant Ad construction
High capacity for (multiple) DNA insertion	Expression is generally transient
Ability to use wide variety of cellular promoters in Ad recombinants	In vivo antivector immune response
High-level protein expression via late Ad regulatory elements	Current Ad vectors cannot be specifically targeted in vivo
Ease of producing high-titer viral stocks
Wide tropism of Ad includes many nondividing cells