Figure 3.
Effect of Media Exchange Frequency on JΔNI5 Vector Production
(A) Timeline of media changes in 4 experimental groups (a–d). Circles in the diagram represent complete media changes. (B and C) Confluent U2OS-ICP4/27 cells in T75 flasks were infected with JΔNI5 virus at an MOI of 10−5 PFU/cell and viral titers in the media were measured daily by quantitative real-time PCR (B) and standard plaque assay (C). (D) gc/PFU ratios were calculated from the results of (B) and (C). Data are presented as means ± SD (n = 3).