Figure 5.
Comparison of Viral Yields under Different SAHA Treatment Conditions
Confluent U2OS-ICP4/27 cells in T225 flasks were treated with 0.5 μM SAHA for 2 h. Cells in the “co-treatment” group were then infected with JΔNI5 virus. Cells in the “pre-treatment” group were washed with PBS and fresh medium without SAHA was added prior to viral infection. All infections were performed with 10−5 viral PFU/cell. (A and B) Fluorescence was recorded at 7 dpi (A) and the titers of supernatant samples were analyzed by quantitative real-time PCR at 9 dpi (B). (C and D) Confluent U2OS-ICP4/27 cells in T225 flasks were treated with the indicated concentrations of SAHA for 2 h and infected with JΔNI5 vector in SAHA-free media. Supernatant samples were collected at 9 dpi and viral titers determined by quantitative real-time PCR (C) and standard plaque assay (D). Differences between pairs were analyzed by Student’s t test for (B), and group comparisons (C and D) were performed with Student’s t test or one-way ANOVA with post hoc Dunnett’s multiple comparison test. Data are presented as the means ± SD (n = 3; ∗p < 0.05, ∗∗p < 0.01 compared to solvent).