Figure 6.
Induction of JΔNI5 Virus Release from Producer Cells by Salts
Confluent U2OS-ICP4/27 cells in T75 flasks were infected with JΔNI5 virus at 10−5 PFU/cell. At 9 dpi, supernatants were replaced with media supplemented with NaCl or CsCl at the indicated concentrations or media alone (control). (A and B) Cultures were then incubated at 33°C for 6 h and supernatants were collected and titered by quantitative real-time PCR (A) and standard plaque assay (B). Group comparisons were performed with one-way ANOVA with post hoc Dunnett’s multiple comparison test. Data are presented as means ± SD (n = 3; ∗p < 0.05 compared to control).