Figure 7.

rDRG Transduction by JΔNI5 Viruses Produced by Optimal and Conventional Protocols

(A) rDRGs were infected with each JΔNI5 virus preparation at 5,000 gc/cell and mCherry fluorescence was photographed at 4 dpi. (B and C) Relative amounts of viral genomes (B) and mCherry mRNA (C) in rDRGs at 4 dpi as determined by quantitative real-time PCR and qRT-PCR, respectively. Intracellular viral genome numbers and mCherry mRNA levels were normalized to cellular 18S rRNA genes and RNA levels, respectively. Differences between pairs were analyzed by Student’s t test. Data are presented as means ± SD (n = 3; ∗∗p < 0.01).