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Summary of Optimal and Conventional JΔNI5 Vector Production Methods
| Optimal Protocol | Conventional Protocol | |
|---|---|---|
| MOI | E-5 | E-4 |
| Temperature | 33°C | 33°C |
| Serum | 0% | 0% |
| Chemical agent | SAHA (0.5 μM, pre 2 h) | none |
| Vector release | NaCl (0.25 M, 2 h) | none |
| Medium collection | days 6, 8, 9 | day 9 |
