Figure 1.

Characterizing the Ischemic Heart Transcriptome and Proteome

(A) Sham-operated heart or heart, 4 or 24 h post-MI was collected, and the ischemic area tissue (or equivalent area in sham hearts) was divided into two equal pieces. One-half of the ischemic heart was sequenced for transcriptomic analysis (n = 10 total, Sham n = 3, 4 h post-MI n = 3, or 24 h post-MI n = 4), whereas the other one-half of the ischemic heart was evaluated for protein level using mass spectrometry (n = 12 total, Sham n = 4, 4 h post-MI n = 4, or 24 h post-MI n = 4). (B and C) Hierarchical clustering dendrogram of 2,272 genes with corresponding mRNA level (B) or 2,397 protein intensity (C) in Sham, 4 h post-MI or 24 h post-MI hearts. (D and E) Correlation analysis between changes in levels of proteins and mRNA in the LV, 4 (D) or 24 (E) h post-MI. The bottom-right shaded rectangles include genes that show static or reduced mRNA levels post-MI, whereas their encoded protein levels increased with comparison to sham. (F and G) A list of genes that encode for proteins with elevated protein levels (fold change > 2), while showing mRNA downregulated (fold change < 0.64), 4 h (F) or 24 h (G) post-MI. Genes with yellow backgrounds have a 5′ UTR that is shorter than 100 base pairs (see Table S1 for the full sequences of the 5′ UTR of the 5 genes).