Figure 5.

Lentiviral Transduction of Human Primary NK Cells

(A) Viability of primary NK cells 48 h after transduction with different compounds at the indicated concentrations. (B) Flow cytometric overlay histogram of GFP expression in NK cells. VSV-G lentivirus was added at the MOI of 10:1 in the presence of 10 μg/mL protamine sulfate. (C) NK cell transduction efficiency was determined by GFP expression in living NK cells. GFP expression was higher after culture with rosuvastatin when compared with DMSO and medium group. (D) Pearson correlation analysis of LDLR MFI (before transduction) and GFP MFI (48 h after transduction) on NK cells. (E) One representative LDLR co-expression with GFP on NK after 48 h viral transduction. Data was showed as mean ± SD from n = 6 independent experiments performed at different times. Data analysis was performed by a two-way ANOVA and Bonferroni post-tests in comparison to medium. For cell viability, Mann-Whitney U test was used to compare with medium group.