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. 2020 Mar 13;11(12):3416–3423. doi: 10.7150/jca.38728

Figure 4.

Figure 4

HOTAIRM1 directly interacts with EZH2 and hinders the deposition of H3K27me3 marks at the putative HOXA1 promoter. (A) RT-PCR and western blot showing RNA and protein levels of EZH2 in MCF7 and tamoxifen-resistant breast cancer (TAMR) cells. (B) RT-PCR and western blot showing RNA and protein levels of EZH2 in HOTAIRM1-knockdown TAMR cells. β-Actin was used as an internal control. (C) RIP-PCR analysis for the interaction between HOTAIRM1 and EZH2 in MCF7 and TAMR cells. U2snRNA was used as a negative control, and HOTAIR was used as a positive control. All experiments were performed in triplicate.