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. 2020 Mar 15;11(12):3492–3501. doi: 10.7150/jca.42070

Figure 2.

Figure 2

SNHG3 deficiency promotes PTC cells proliferation in vitro. (A) RT-PCR confirmed the knockout efficiency of SNHG3 by CRISPR/Cas9 technology in the BCPAP and TPC-1 cells. (B) CCK-8 assays were performed to verify the functional role of SNHG3 in BCPAP and TPC-1 cell viability. (C) Representative graphs and quantification results of colonies in colony formation assay. (D) Knock-out of SNHG3 accelerated cell proliferation in PTC cell lines as determined by EdU assays. Error bars represent means ± SD from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.