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. 2020 Mar 15;11(12):3492–3501. doi: 10.7150/jca.42070

Figure 5.

Figure 5

mTOR inhibitor AZD8055 impedes SNHG3-mediated PTC cell growth, migration and invasion. (A) BCPAP cells from control and SNHG3-KO group were treated with DMSO or 500 nM AZD8055 for 48h and the cell viability was detected by CCK-8 assays. (B) The quantification of colonies per well was counted and analyzed from PTC cells in the presence or absence of AZD8055. (C) Transwell experiments showing the effects of SNHG3 on BCPAP cell migration and invasion following treatment with AZD8055 for 48h. (D) Western blotting analysis of the protein levels of mTOR, AKT, ERK and their phosphorylation forms in BCPAP control and knockout cells with AZD8055 for 48h. (E) The densitometric analysis results of the western blot bands shown in Fig. 5D. Error bars represent three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.