Figure 2.

METTL3 promoted PCa cell proliferation, migration, and invasion dependent on its m6A methylase activity. (A) The interference efficiency of METTL3 in C42 cells. (B) The effect of METTL3 knockdown on the m⁶A contents of poly(A) RNAs in C42 cells. (C-E) The effect of METTL3 knockdown on C42 cells proliferation (C), migration (D), invasion (E). (F) The over-expression efficiency of METTL3 in PC3 cells. (G) The effect of METTL3 over-expression on the m⁶A contents of poly(A) RNAs in PC3 cells. (H-J) The effect of METTL3 over-expression on the PC3 cells proliferation (H), migration (I), invasion (J). K: Western blot confirmation of wild-type of METTL3 and catalytic mutant form in PC3 cells. L: The effect of wild-type METTL3 and catalytic mutant METTL3 on the m⁶A contents of poly(A) RNAs in PC3 cells. (M-O): The effect of wild-type METTL3 and catalytic mutant METTL3 on the PC3 cells proliferation (M), migration (N), invasion (O).