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. 2020 Mar 26;11(12):3623–3633. doi: 10.7150/jca.37393

Figure 6.

Figure 6

HEY1 is a key factor in the tumorigenicity of 143B osteosarcoma cells. (A) Protein expression level of HEY1 in six osteosarcoma cell lines (MG-63, Saos-2, U-2 OS, MNNG/HOS, 143B, and SJSA-1). (B) Protein expression level of HEY1 after lentivirus-mediated RNA interference in 143B osteosarcoma cells. (C) Lentivirus infection efficiency detected by fluorescence microscopy (scale bar = 200 μm). (D) Lentivirus-infected 143B osteosarcoma cell pellet under visible light. (E) Images of 143B cell-bearing mice under 530-nm laser irradiation at the experimental endpoint. Subcutaneous tumors at the experimental endpoint (F), tumor growth curves (G), and tumor weight (H) are shown. (I) Protein expression level of HEY1 in Saos-2 and HEY1-overexpressed Saos-2 cells. (J) Tumorigenicity assay of Saos-2 cells after HEY1-overexpression. Subcutaneous tumors at the experimental endpoint (K), tumor growth curves (L), and tumor weight (M) of Saos-2 cells after HEY1-overexpression are shown.