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. 2020 Mar 13;11(12):3454–3466. doi: 10.7150/jca.41418

Figure 2.

Figure 2

Niclosamide significantly represses mitochondrial respiration and interrupt mitochondrial dynamics. A-D. The intact cellular oxygen consumption rate (OCR) of Niclosamide treated SKOV3 and HO8910 cells under the indicated conditions were measured in real time using the Seahorse XF96 Extracellular Flux Analyzer. Basal OCR were measured at three time points, followed by sequential injections of the ATP synthase inhibitor oligomycin (1 μM), the uncoupler FCCP (1 μM), the complex I inhibitor rotenone (1 μM), and the complex III inhibitor antimycin A (1 μM). E. The mitochondrial morphology in Niclosamide treated SKOV3 and HO8910 cells were examined by DAPI and MitoTracker Red. F. Western blot analyses of OPA1 and OMA1 in Niclosamide treated SKOV3 and HO8910 cells. Actin was used as a loading control. G. SKOV3 and HO8910 cells were treated with or without Niclosamide (4, 8μM) for 24 hr. Cell samples were collected and stained with JC-1 MMP detection kit and analyzed by flow cytometry.