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. 2020 Mar 13;11(12):3454–3466. doi: 10.7150/jca.41418

Figure 4.

Figure 4

Niclosamide promotes cell apoptosis effectivelly under the condition of glucose deprivation. A. Flow cytometry analysis of cell apoptosis after the ovarian carcinoma cells treated with Niclosamide in the presence or absence of glucose for 12 hr. Cells were collected and stained with Annexin V-fluorescein isothiocyanate (FITC) and PI. Data are presented as mean ± SD. B. SKOV3 cells were treated with Niclosamide in the presence or absence of glucose for 12 hr, then subjected to culture for 3 days. Representative images of colonies and total colonies were recorded and calculated. Data are presented as mean ± SD. C. Western blot analysis of p-JNK and JNK in Niclosamide treated SKOV3 cells in the presence or absence of glucose for 12 hr. Actin was used as a loading control. Data are presented as mean ± SD. D. Flow cytometry analysis of cell apoptosis after the ovarian carcinoma cells were treated with Niclosamide and/or NAC in the presence or absence of glucose for 12 hr. Cells were collected and stained with Annexin V-fluorescein isothiocyanate (FITC) and PI. Data are presented as mean ± SD. E. SKOV3 cells were treated with Niclosamide and/or NAC in the presence or absence of glucose for 12 hr, and then cultured for 3 days. Representative images of colonies and total colonies were recorded and calculated. Data are presented as mean ± SD. F. Western blot analysis of p-JNK and JNK in Niclosamide treated SKOV3 cells with or without NAC and in the presence or absence of glucose for 12 hr. Actin was used as a loading control. Data are presented as mean ± SD.