Figure 4.

Multiple targets of miR-186. (A) The predicated targets PIK3R3, PTEN and APAF1 were showed in bioinformatics database. (B) HEK 293T cells were co-transfected with miR-186mimic or NC and a luciferase recombinant with the 3'-UTR of PIK3R3, or APAF1, or PTEN, then the relative luciferase activity was detected 24 hours later. (C) The protein levels of PIK3R3, APAF1 and PTEN were measured by western blotting 48 hours after transfection with miR-186 mimic or NC in A2780/DDP cells. (D) The quantitative analysis of protein levels (normalized to GAPDH) by Image J. *P<0.05.