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Multiplex immunofluorescence in HLRCC type. A. DAPI nuclear stain. B. PD-L1 positive in green color. C. PD-1 positive in magenta. D. CD8+ in white. E. Combination of markers without unmixing colors; note positive staining of PD-L1 at the tumor area (green) and peritumoral distribution of cells co-expressing CD8+ and PD1+ (pink color: magenta and white overlapped).
