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. 2020 Mar 13;11(12):3476–3482. doi: 10.7150/jca.29751

Figure 2.

Figure 2

ATO induced autophagy in the FLT3-ITD AML cell line. (A) MV4-11 cells were treated with ATO for 24 h, and the expression levels of LC3, ATG5 and ATG7 were detected by western blot analysis. GAPDH expression was used as a loading control. (B) MV4-11 cells transfected with mRFP-GFP-LC3 plasmids were treated with ATO for 0, 12, and 24 h and then observed by confocal fluorescence microscopy. Scale bar=5 µm. (C) MV4-11 cells were treated with ATO for 0, 12, and 24 h. The number of autophagosomes was observed by transmission electron microscopy and calculated (each group had 30 views, * P<0.05, ** P<0.01). Black arrows represent autophagosomes or autophagolysosomes. Scale bar=2 µm.