Figure 3.

Degradation of FLT3-ITD by ATO was reversed by inhibition of autophagy (A). MV4-11 cells were treated with ATO for 0, 12, and 24 h, and immunofluorescence staining of p-FLT3 was analyzed. (B). MV4-11 cells were treated with ATO alone or in combination with bafilomycin A (BafA) for 24 h, and p-FLT3 and LC3-I to LC3-II conversion were assessed by western blot analysis. (C). MV4-11 cells were transfected with siRNAs targeting Atg5 or Atg7 for 48 h and then treated with or without ATO for 24 h. Then, p-FLT3, ATG5 and ATG7 were assessed by western blotting. GAPDH expression was used as a loading control.