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. 2020 Mar 26;11(12):3655–3666. doi: 10.7150/jca.42771

Figure 5.

Figure 5

Per2 overexpression inhibits proliferation and promotes apoptosis and autophagy in OSCC cells via the PI3K/AKT/mTOR signaling pathway. (A) TEM experiments showed that the elevated autophagosome density was obviously decreased significantly after the addition of SC79 in Per2-OE cells (low magnification scale bars = 2 μm; high magnification scale bars = 1 μm). (B) Western blotting indicated that, following the addition of SC79, the level of p-mTOR was increased; moreover, the LC3B II/I ratio was significantly decreased, while p62 and Beclin1 protein expression was significantly elevated. (C) The apoptosis index as measured by flow cytometry was significantly reduced after the addition of SC79 to Per2-OE cells. All data represent three independent experiments. (D) Flow cytometry indicated that the proliferation percentage of Per2-overexpressing TSCCA cells was upregulated as a result of SC79 addition. Data are presented as the mean ± SD (n ≥ 3). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.