Figure 3.
miR-365a-3p impairs CRC invasion and metastasis. (A) Transwell-based invasion and migration assays were used to assess the impact of miR-365-3p overexpression on HCT116 and SW620 cell migratory and invasive activity, with representative images shown. (B) Data from A, quantified as means ± SEM from triplicate experiments. (C) Levels of E-cadherin, N-cadherin, and vimentin were measured via Western blotting in CRC cells overexpressing miR-365a-3p. (D) Nude mice were administered a tail vein injection of 4×105 HCT116 cells expressing either NC or miR-365-3p (n=9 mice), and after 10 weeks the number of lung metastases in each animal was assessed via microscopic examination, with representative H&E stained images shown (Upper:×40; Lower: ×100) (E) Numbers of lung metastases were quantified as means ± SEM. (F) Overall survival for mice in each group was assessed via the Kaplan-Meier approach. *p < 0.05; **p < 0.01; ***p < 0.001.