Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Mar 26;11(12):3634–3644. doi: 10.7150/jca.42731

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The author(s)

This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.

PMC Copyright notice

miR-365a-3p directly targets ADAM10 in CRC. (A)Three bioinformatics tools were used to identify potential miR-365a-3p targets. (B) The 81 target genes that overlapped among these predictive tools were validated via qRT-PCR in CRC cells. (C) miR-365a-3p and the corresponding predicted binding site in the 3'-UTR of ADAM10, with wild-type (WT) and mutated (Mut) versions of this sequence being shown. (D) A clear reduction in luciferase activity was evident in CRC cells overexpressing miR-365-3p and the WT but not the mutated version of the ADAM10 3'-UTR. (E) Overexpression of miR-365a-3p in CRC cell lines reduced levels of ADAM10 protein. UTR: untranslated region. *p < 0.05; **p < 0.01; ***p < 0.001.